By Anthony Bly, SDSU Extension Soils Field Specialist

Crop production has successfully relied on inorganic agronomy (NPK based knowledge) for decades. Basically, inorganic agronomy was the more easily available science to discover and returned measurable crop production benefits during this time. As scientific processes developed and detection limits increased from parts per million (ppm) to parts per billion (ppb) and even trillion in some cases, new and emerging areas of science have been discovered. The natural progression of science is to investigate deeper and deeper into the unknown. The soil is a new frontier for scientific discovery. For decades scientists have known that a handful of soil contained more micro-biological organisms than the number of humans on earth. Science is just beginning to discover these organisms and learn about their functions and contribution to their soil ecosystem.

Why does this matter?

Many of the soil organisms influence crop access and uptake of essential plant nutrients. Carbon is a common link or foundation for all of the microbial life in the soil. We know that as we build soil organic carbon, the nutrient cycling of essential plant nutrients improves. Soil physical and chemical properties improve which creates a more favorable environment for soil microbial life and plant roots. Much research has been conducted on the effects of tillage on soil microbiology. The benefits to stopping tillage are perceived as somewhat elusive to producers and most delay adoption due to short land tenures. Researchers at SDSU conducted an evaluation comparing microbiology communities of short and long term no-till crop fields to conventionally tilled fields in Eastern South Dakota.

Left: Soil from long-term no-till field. Right: Soil from conventionally managed field that included tillage and crop residue removal.

What was done

Four locations near Beresford, Mitchell, Crooks and Garretson were identified where long-term and short-term no-till fields were compared to conventionally tilled. The fields were located in close proximity to each other and for most all of the comparisons, the fields were across a fence/property line. Long-term no-till plots ranged from 20 to 26 years in no-till management and short-term were 3 to 5 years. Conventional tillage systems included a Fall chisel plowing and spring field cultivation passes. During August-September, composite soil samples (0-3 inches) were obtained from 4 replications in each field. The samples were analyzed for 8 different enzymes, bacteria, fungi, organic carbon and nitrogen.


Most all of the measured enzymes at each location were significantly higher in the long-term no-till fields. The short-term no-till fields had higher enzyme levels when compared with the conventionally tilled fields of which nearly 50% of the comparisons were not different from the long-term no-till fields. The bacteria and fungi levels were also higher in the long-term no-till fields when compared to conventionally tilled fields of which nearly 50% of the short-term no-till fields were similar to long-term no-till. Cold and hot water carbon and nitrogen were not significantly different between the tillage systems. However, microbial carbon and nitrogen were significantly higher for the long-term no-till at 3 of the 4 locations. The short-term no-till microbial nitrogen and carbon were similar to the long-term no-till at 2 of the 4 locations.


  • No-till significantly increased soil biological health when compared to conventional tillage.
  • Short-term no-till had very positive increases just after 3-5 years.
  • In-organic agronomy is still need for soil nutrient management.


Sekaran, Udayakumar, Kavya Laxmisagra Sagar, Luiz Gustavo De Oliveira Denardin, Jaseep Singh, Navdeep Singh, Gandura Omar Abagandura, Sandeep Kumar, Bhupinder Singh Farmaha, Anthony Bly and Amanda Posselt Martins. 2019. Response of soil biochemical and microbial community structure to short and long-term no-till systems. European Journal of Soil Science (in publication).