Source: Kansas State University Extension Agronomy

(Note: The following is an excerpt from Glyphosate Stewardship: Optimizing and Preserving Glyphosate Performance, a new K-State Research and Extension publication, MF-2767. This publication is available online at:

Nov. 26, 2013 — Environmental conditions influence the activity and weed control achieved with any herbicide. Optimal conditions for plant growth usually result in the best weed control and the least risk of crop injury. Any environmental conditions that cause plant stress may reduce herbicide uptake and performance. 

Environmental factors that may influence weed control with glyphosate in particular include drought, temperature, relative humidity, presence of dew, rainfall following application, and light. 

Weed control and the speed of control with glyphosate generally increase as air temperatures increase, as long as the plant is still actively growing. However, high temperatures that place plants under stress, or when combined with dry conditions, likely will result in reduced control. Glyphosate applied during cool periods before, during, or after treatment will result in a slower plant response and may result in decreased control. 

Herbicide absorption and weed control generally are greater with higher humidity levels. Lower humidity conditions, which are common in the Great Plains, may result in reduced herbicide absorption and control. Relative humidity levels often fluctuate with the time of day, being highest at night and in the morning and lowest during the midday hours. 

High nighttime and early morning humidity may result in heavy dew, which may have an adverse effect on control. These conditions may reduce glyphosate activity by increasing spray droplet runoff and/or decreasing the concentration of glyphosate in the spray solution on the leaf surface. 

Rainfall soon after an application can wash herbicide off plant foliage and reduce weed control. The interval required between application and rainfall depends on a number of factors. Consult product labels for recommended intervals between application, and pay close attention to rainfall forecasts. 

Changes in light with the time of day also seem to influence control. Even though environmental conditions may seem more favorable in the morning or evening, research at several universities has demonstrated that weed control with glyphosate is often lower early in the morning or in the evening as compared to midday applications (Figures 1 and 2). This response is species dependent. Weeds such as volunteer wheat tend not to be adversely affected by applications in late evening or early morning.

Environmental Factors Affecting Glyphosate Performance

Figure 2. Velvetleaf control was much better when glyphosate was applied at 10 a.m. than at 9 p.m. in this  K-State test. Photo by Dallas Peterson, K-State Research and Extension.Figure 2. Velvetleaf control was much better when glyphosate was applied at 10 a.m. than at 9 p.m. in this K-State test. Photo by Dallas Peterson, K-State Research and Extension.

This phenomenon is not completely understood, but several possible contributing factors include interactions with dew, diurnal leaf movement patterns, and interactions with plant physiological processes. Some plants, such as velvetleaf and morningglory, fold their leaves down at night. Consequently, if the leaves are more vertical when the herbicide is applied, there is less spray coverage and herbicide uptake by the plant resulting in less control. 

Fluctuation in control due to application time seems to correlate closely with light intensity and daily plant photosynthetic cycles. Glyphosate absorption, translocation, and metabolic activity may be affected by the photosynthetic cycle. 

Light seems to be the key factor in the effect of time of day on glyphosate activity. Applications after sunrise and before the sun starts to set have generally provided the best control. Wind also is an important consideration: spray to avoid herbicide drift. 

Consult with local weather forecasts and plan applications to minimize drift and optimize herbicide performance.